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Edinburgh Genomics: Introduction to Python for Biologists

Python is a dynamic, readable language that is a popular platform for all types of bioinformatics work, from simple one-off scripts to large, complex software projects. This workshop is aimed at complete beginners and assumes no prior programming experience. It gives an overview of the language with an emphasis on practical problem-solving, using examples and exercises drawn from various aspects of bioinformatics work. The workshop is structured so that the parts of the language most useful for bioinformatics are introduced as early as possible, and that students can start writing plausibly-useful programs after the first few sessions. After completing the workshop, students should be in a position to (1) apply the skills they have learned to tackling problems in their own research and (2) continue their Python education in a self-directed way.

 Instructor:

Tim Booth

Course Developer:
Dr. Martin Jones (Founder, Python for Biologists)

Workshop format
Five days. See web page for details 

Overview

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Researchers needing to implement data analysis workflows face a number of common challenges, including the need to organise their tasks, make effective use of compute resources, handle unexpected errors in processing, and document and share their methods. The Snakemake workflow system provides effective solutions to these problems. By the end of the course, you will be confident in using Snakemake to tackle complex workflow problems and in your day-to-day research.

Who this course is for
This course is intended for researchers who need to automate data analysis tasks for biological research involving next-generation sequence data, for example RNA-seq analysis, variant calling, CHIP-Seq, bacterial genome assembly, etc. Attendees must have a working knowledge of how to use the Linux BASH command line - our 1-day "Linux for bioinformatics" course is a suitable background. The language used to write Snakemake workflows is Python-based, but no prior knowledge of Python is required or assumed.

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The aim of this course is to introduce participants to the statistical computing language 'R' using examples and skills relevant to biological data science. This online workshop is taught by experienced Edinburgh Genomics’ trainers. By the end of the workshop, you will be comfortable with the basics of the R and R studio environments, learning about the rules of the language and how R works with different data types and structures. We then move on to using functions, introducing a selection of packages for biological data science. Finally, we will learn how to visualise your data to generate publication-ready plots using the package ggplot2.

Instructors

• Frances Turner (Bioinformatician)
• Heleen De Weerd (Bioinformatician)

Workshop format

The workshop consists of presentations and hands-on tutorials.

Who should attend

Undergraduates, Graduates, postgraduates, and PIs, who are using, or planning to use, the statistical software R to manipulate and analyse biological data in their research. This is an introductory level course: no prior experience of R (or any other programming language) is necessary before starting the workshop.

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This course aims to provide an introduction to the principles of short variant discovery (both germline and somatic) from short-read data. We will look at a complete workflow, from data QC to functional interpretation of variant calls. The practical sessions will focus on running the GATK pipeline from the Broad Institute.

Instructors: Frances Turner & the Edinburgh Genomics Bioinformatics Team

Venue: Online

Requirements:

This course is intended for researchers who need to analyse genomic data in order to call genomic variants. Aside from a basic understanding of molecular biology, attendees must have a working knowledge of how to use the Linux BASH command line - our 1-day "Linux for bioinformatics" course is a suitable background.

PLEASE SIGN UP HERE BEFORE PAYING FOR THIS COURSE

Whole-genome sequencing (WGS) has been revolutionised by the development of long-read sequencing technologies in the last few years. Driven in no small part by Oxford Nanopore Technologies (https://nanoporetech.com/), we now have the ability to sequence long (kb+) single-molecule DNA fragments. Although these developments are expected to alleviate numerous computational challenges surrounding genome assembly they also bring some interesting bioinformatics challenges to which we have to adapt in order to get the most from this powerful technology.

This exciting course aims to introduce the principles and practice of long-read genome assembly and analysis using cutting-edge software and best practices tried and tested by our expert bioinformaticians here at Edinburgh Genomics.

Who this course is for

Aside from a basic understanding of molecular biology, attendees must have a working knowledge of how to use the Linux BASH command line - our 1-day "Linux for bioinformatics" course is a suitable background.

PLEASE SIGN UP HERE BEFORE PAYING FOR THIS COURSE

Edinburgh Genomics: DNA Library Preparation Lab Course 

In this course you will learn how to produce short-read DNA sequencing libraries using the NEBNext UltraExpress™ FS DNA Library Prep Kit which provides a simple and robust ~2 hourwork-flow for library production. You will get to work on a high-quality training sample or have the opportunity to bring your own DNA sample for library preparation and sequencing. This course assumes you have a basic level of laboratory experience in a molecular biology lab, although experienced demonstrators will always be on hand to guide you. If you have limited lab experience, please indicate this on the registration form and we will try to accommodate you.

Who should attend: This workshop is aimed at researchers & technical workers with a background in biology who intend to use their skills in a molecular biology lab.

Requirements

That you attend all parts of the course.

PLEASE SIGN UP HERE BEFORE PAYING FOR THIS COURSE

Edinburgh Genomics: Illumina 

In this course you will learn how to produce whole genome sequencing libraries using the new RNA library preparation kits from Illumina, which provide a quick and simple work-flow for library production. You will get to work on both a high-quality training sample and have the opportunity to bring your own RNA sample for library preparation and sequencing. This course assumes you have a basic level of laboratory experience in a molecular biology lab, although experienced demonstrators will always be on hand to guide you. If you have limited lab experience, please indicate this on the registration form and we will try to accommodate you.imple work-flow for library production. More detailed description of the session can be found on the pdf you receive when signing up.

Who should attend: This workshop is aimed at researchers & technical workers with a background in biology who intend to use their skills in a molecular biology lab.

Requirements

That you attend all parts of the course.

PLEASE SIGN UP HERE BEFORE PAYING FOR THIS COURSE

Edinburgh Genomics: RNA-seq Data Analysis

RNA sequencing (RNA-seq) has become the method of choice for transcriptome profiling. Nevertheless, it is a non-trivial task to transform the vast amount of data obtained with high-throughput sequencers into useful information. Thus, RNA-seq data analysis is still a major bottleneck for most researchers in this field. The ability to correctly interpret RNA-seq results, as well as knowledge of the intrinsic properties of these data, are essential to avoid incorrect experimental designs and the application of inappropriate analysis methodologies. The aim of this workshop is to familiarise researchers with RNA-seq data and to initiate them in the analysis by providing lectures and practicals on analysis methodologies. In the practicals Illumina-generated sequencing data and various widely used software programs will be used.

Instructors: Urmi Trivedi, Frances Turner, Nathan Medd 

Workshop format

The workshop consists of presentations and hands-on tutorials.

Who should attend

Graduates, postgraduates, and PIs, who are using, or planning to use, RNA-seq technology in their research and want to learn how to process and analyse RNA-seq data.

Requirements

A general understanding of molecular biology and genomics. Experience of command-line computing and a working knowledge of R at the level of Edinburgh Genomics introductory courses.

PLEASE SIGN UP HERE BEFORE PAYING FOR THIS COURSE

Edinburgh Genomics: RNA-seq Data Analysis

RNA sequencing (RNA-seq) has become the method of choice for transcriptome profiling. Nevertheless, it is a non-trivial task to transform the vast amount of data obtained with high-throughput sequencers into useful information. Thus, RNA-seq data analysis is still a major bottleneck for most researchers in this field. The ability to correctly interpret RNA-seq results, as well as knowledge of the intrinsic properties of these data, are essential to avoid incorrect experimental designs and the application of inappropriate analysis methodologies. The aim of this workshop is to familiarise researchers with RNA-seq data and to initiate them in the analysis by providing lectures and practicals on analysis methodologies. In the practicals Illumina-generated sequencing data and various widely used software programs will be used.

Instructors: Urmi Trivedi, Frances Turner, Nathan Medd 

Workshop format

The workshop consists of presentations and hands-on tutorials.

Who should attend

Graduates, postgraduates, and PIs, who are using, or planning to use, RNA-seq technology in their research and want to learn how to process and analyse RNA-seq data.

Requirements

A general understanding of molecular biology and genomics. Experience of command-line computing and a working knowledge of R at the level of Edinburgh Genomics introductory courses.

PLEASE SIGN UP HERE BEFORE PAYING FOR THIS COURSE

Edinburgh Genomics: Introduction to Linux for Genomics

Genomic studies produce vast amounts of data, usually in the form of very large text files. Linux is particularly suited to working with such files, and is therefore arguably one of the most important tools in a bioinformatician’s toolkit. The Linux command-line enables one to view,
filter and manipulate large text files that are difficult or impossible to handle with applications like Word or Excel, write pipelines to mperform certain tasks, and run bioinformatics software for which no web interface is available. In this workshop, we will first cover the most used Linux commands, followed by a short introduction to several popular command-line tools that were specially developed for genomics as well as file formats commonly used in genomics (BED, FASTA, FASTQ, GFF/GTF, SAM/BAM, VCF).

Instructor: Tim Booth

Workshop format
The workshop consists of guided tutorials and hands-on exercises. Roughly 3/4 of the workshop will be spent on Linux and 1/4 on command-line tools for genomics and file formats.

Who should attend
Graduates, postgraduates, and PIs, without any previous command-line experience.

Requirements
A general understanding of molecular biology and genomics and elementary skills in computer usage are required. A computer with stable internet connection and small VNC viewer software (download instructions included)